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Some of the clinocal features such as chronic infections, recurrent infections and incomplete responses to chemotherapy often provide an important clue to establish antibody (B cell) immunodeficiency diseases mainifest. Early diagnosis of hypogammaglobulinemia or demostration of marked deficiency of immunglobulin in the patients who have such clinical features is of utmost importance in the management or treatment of patients with administration of either gamma-globulin or specific immunoglobulins. A number of screening tests have been recently developed in the diagnosis of specific immunodeficiency disorders. Among the functional evaluation of antibody-mediated immunity aer protein electrophoresis, radial immunodiffusion test, Schick test, isohemagglutinin test, specifc antibody response and B cell quantitation. A demostration of either isohemagglutinin which resultedfrom immunization with natural A and B substances or bacterial agglutinins to Propionibacterium acnes which comprises the predominant resident flora of the skin may be useful since they would provide immediate results without any specific immunizations onto the patients. However, as a screening test of antibody-mediated immunity in healthy individuals, the significance of isohemagglutinin in test and bacterial agglutination test with P. acnes has not been evaluated for their sensitivities. In this study, titres of isohemagglutinin(IgM class) and bacterial agglutinins to P. acnes(IgG class) were measured among ABO blood groups by means of microtitration technique. In the sera of O group, anti- A isohemagglutinin titre ranged from log₂1(25.0%) to log₂8(8.3%) and its mean titre was log₂3.41 2.22, and anti-B isohemagglutinin titre ranged from log₂1(41.6%) to log₂5(16.6%)and its mean titre was log₂2.40±1.40, and bacterial agglutinin titre to P. acnes ranged from log₂4(20.2%) to log₂7(20.0%) and its mean titre was log₂5.60±1.04. In the B group, anti A isohemagglutinin titre ranged from log₂1(16.6%) to log₂4(33.3%)and its mean titre was log₂2.66±1.15, and bacterial agglutinin titre aganist P. acnes ranged from log₂5(16.6%) to log₂7(33.3%)and its mean titre was log₂6.16±0.71. In the AB blood group, bacterial agglutinin titre ranged from log₂3(16.6%) to log₂8(16.6%)and its mean titre was log₂5.58±1.28. In the O blood group, anti-A titre was greater than anti-B titre(P<0.05). There were no significamt differences in anti-A titres between O and B groups and in anti-B titres between O and A group. Bacterial agglutinin titre of O group was higher than those of A group(P<0.05) and AB group(P<0.01). Isohemagglutinin titre(IgM class) and bacterial agglutinin titre(IgG class) showed no correlation in the microtitration system. These results indicate that bacterial agglutinins to P.acnes(IgG class)is higher than Isohemagglutinin(IgM class) in normal human serum but both tests may be useful independently for selective immunoglobulin deficiency test.
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